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Related post: studies. We have demonstrated Buy Cheap Terramycin that cytoplasmic mRNAs produced Generic Terramycin by small numbers of cultured cells can be detected and quantitated by sensitive dot blot assays using class and allotype-specific probes. PHS6040 (Rev. 1/83) 20-17 GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES • PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00037-16 LI PERIOD COVERED October 1, 1982 to September 30, 1983 TITLE OF PROJECT (80 characters or leu. Title mu$t fit on one line between the borders.) Immunogenetics of Mouse Immunoglobulins and Genetic Control of Antibody Response PRINCIPAL INVESTIGATOR (List other professional personnel on tutMcquent pages.) (Name, title, laboratory, and institute affiliation) Rose Lieberman, Senior Investigator, Laboratory of Immunology, NIAID COOPERATING UNITS (if any) M. Potter, LCB/NCr, L.A. D'Hoostelaire, Litton Bionetics, Rockville, MD. J.M. Dome, Washington University School of Medicine, St. Louis, MO. and F. Wrener, Karolinska Institutet, Stockholm, Sweden G. Klein, LAB/BRANCH Laboratory of Immunology INSTITUTE AND LOCATION ^IIAID, Building 10, Room 11N322 TOTAL MANYEARS: 3 PROFESSIONAL; 2 CHECK APPROPRIATE BOX(ES) □ (a) Human subjects □ (a1) Minors □ (a2) Interviews □ (b) Human tissues C3 (c) Purchase Terramycin Neither SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.) Identification of mice that have altered responses to phosphoryl Buy Terramycin Online choline (PC) antigens will facilitate the understanding of the genetic control of immune responses . Most inbred strains produce anti-PC antibodies of limited heterogeneity involving three dominant V.-Vm pairs. In the BALB/c however, anti-PC antibodies are predominantly of the VmT15-V|.22 idiotype and at the DNA level have been shown to be controlled by a single gene. In other strains there are equal proportions of anti-PC antibodies of the three clonotypes. It has been shown that anti-PC response is regulated by both cellular and humoral mechanisms such as recognition of idiotypes by T. MHC and T. Id helper cells or by suppression of anti-Id antibody resulting Tn an altered anti-PC response. Recently Purchase Terramycin Online a population of wild mice designated CNV Order Terramycin Online were found which failed to make anti-PC antibodies upon immunization with various PC containing antigens. When CNV mice were mated to C.B20, an inbred responder strain, 82% of the Buy Terramycin F, failed to give an anti-PC responses suggesting a dominant PC gene of CNV origin. Analysis of Fp progeny of the PC hybrids showed that mice homozygous for the CNV allotypes were PC whereas F„ mice (CNV x C.B20) heterozygous for ^oth allotypes and mice homozygous for C.B20 allotype expressed both PC and PC phenotypes suggesting a second regulatory gene that segregates independently and is Order Terramycin not linked to allotype. PHS 6040 (Rev. 1/83) 20-18 GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00040-09 LI PERIOD COVERED October 1, 1982 to September 30, 1983 TITLE OF PROJECT (80 charoctert or less. Title Terramycin Online must fit on one line between the borders.) Genetic control of immunocompetent cell interactions PRINCIPAL INVESTIGATOR (Liet other professional personnel on subsequent pages.) (Name, title, laboratory, and institute affiliation) Ethan M. Shevach, M.D., Sr. Investigator, LI, NIAID COOPERATING UNITS (if any) LAB/BRANCH Laboratory of Immunology INSTITUTE AND LOCATION NIAID, NIH, Bethesda, Maryland 20205 TOTAL MANYEARS: 3.5 PROFESSIONAL: 2.0 1.5 CHECK APPROPRIATE BOX(ES) □ (a) Human subjects □ (a1) Minors □ (a2) interviews □ (b) Human tissues [XI (c) Neither SUMMARY OF WORK (Use standard unreduced type. Do not exceed the ipace provided.) The goals Of this project are to determine the role of I- region associated (la) antigens in the regulation of immunocompetent cell interactions and to determine the mechanism of action and site of expression of the major histocompatibility linked immune response (Ir) genes . We have analyzed the immune responses of guinea pig T lymphocytes which have been primed in vitro with allogeneic bovine insulin pulsed macrophages and have demonstrated that strain 2 macrophages were fully competent to present bovine
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